Please read the following instructions in order to learn how to do this.

For all Descriptions - please use the column links on the experiment submission form to see previous entries.  Adhere to the style of these entries as closely as possible.  Please do NOT use commas.

From the main screen click:

Experiments and Results

(feel free to click on the picture for a larger version)

Select you Organism and press:

Enter Experiment(s) into LMD

Remember, your data files must be in your incoming directory in order for the file drop-downs to be populated.

Slide Name:

This should use the name of the array and the number of the chip.

Our arrays have an organism prefix (HS) and the print batch number, which should be followed by the slide number. Duplicate submissions of the same data is OK, but the names should be identical and distinguished with a number. Please avoid spaces and commas altogether.

Good
1. HS1-221
2. HS1-221-2
3. HS1-222
4. SC6-031

Bad & Ugly
1. HS1 221
2. HS1-221-b
3. HS-1 222
4. SC6_031

Note that the TIF files must be separate images for both the red and green channels.  If you have a combined TIF image you will need to get the separate channel images by extracting them in GenePix®.

Need help doing this for lots of files?

The idea is to make it as descriptive in as few words as possible. Consistency in naming a series of experiments is useful so I suggest opening an additional browser window to see the list of what you've already entered. Include what's in each of the two channels, adopting the custom of green channel first. Include other key information that is not redundant.

If a series of numbers is used, entering them with leading zeros is useful for sorting. Examples for the same series of experiments:

Good
1. Glucose vs. Galactose 100 ng total RNA amplified
2. Glucose vs. Galactose 200 ng total RNA amplified
3. Glucose vs. Galactose 500 ng total RNA amplified
4. Heat shock (Ref. vs. 37 C) 05 min
5. Heat shock (Ref. vs. 37 C) 30 min

Bad & Ugly
1. Glucose vs. Galactose 100 ng total RNA amplified
2. Glucose vs Galactose 200 ng total, amp.
3. Glu vs. Gal, 0.5 microgram, total RNA, amplified
4. Yeast Ht. shock (Ref. vs. 37 C) 5 min.
5. Yeast Heat-shock (Ref. vs. 37) 1/2 hour

Experiment Description:

It's a free text field so anything goes. Copy paste from previous descriptions is OK. Include distinguishing keywords.

eg: Pooled reference/Amplified genomic DNA

Red Channel Description

eg: ABC-3xHA IP 37 C 30 min

Again, succinct and informative, as suggested in the examples.

Experiment Access

For now, please select NO to the question of whether you want your experimental data to be viewable by all users.

Unless you have a very specific reason, please keep ALL selected as a group that can view your experimental data. 

You will most likely wish to also select your group as well (using the CTRL key to select multiple groups).

Press the button:

Load Experiment into LMD

A link is provided for you to monitor the progress of the experiment submission.

You are done!

It is now time to view and analyze your data.